精品国产乱码一区,欧美自拍网站一二,亚洲AV熟女传媒一区二区三区,亚洲在线麻豆

咨詢熱線

15000266580

當前位置:首頁 >產(chǎn)品中心>細胞庫>豬腎細胞系>CCL-33 PK-15 豬腎細胞系

CCL-33 PK-15 豬腎細胞系

簡要描述:CCL-33 PK-15 豬腎細胞系,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件

  • 產(chǎn)品型號:
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2025-04-27
  • 訪  問  量:4320

產(chǎn)品分類

Product Category

詳細介紹

CCL-33 PK-15 豬腎細胞系,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻優(yōu)培養(yǎng)條件

CCL-33 PK-15 豬腎細胞系 的詳細介紹


ATCC® Number:CCL-33™  Price:$355.00
Designations:PK(15)

Depositors:Cutter Laboratories, Inc.

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Sus scrofa (pig)

Morphology:epithelial

Source:Organ: kidney
Disease: normal


Cellular Products:plasminogen activator; keratin

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Applications:transfection host (Roche FuGENE® Transfection Reagents)

Virus Resistance:poliovirus 2

Age:adult

Comments:The presence of a porcine papovavirus in PK(15) cells has been reported in cells obtained from multiple sources including the ATCC . [53398] [53399]

The Foreign Animal Disease Diagnostic Laboratory of the US Department of Agriculture has determined that ATCC CCL-33 is not infected with Hog cholera virus or African swine fever virus, and uses this line to screen for those viruses. [105588]

The cell line harbors an endogenous C-type retrovirus. [26185] [53399] [56104]

The cells are positive for porcine circovirus (PCV) antigens.

The cells are positive for keratin by immunoperoxidase staining.



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C


Subculturing:Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week

Rinse the cell sheet 2 times with fresh 0.25% trypsin, 0.03% EDTA solution, remove trypsin and allow the culture to stand at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.



Preservation:culture medium 95%; DMSO, 5%

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020



References:22988: Dulac GC, Afshar A. Porcine circovirus antigens in PK-15 cell line (ATCC CCL-33) and evidence of antibodies to circovirus in Canadian pigs. Can. J. Vet. Res. 53: 431-433, 1989. PubMed: 2686830

26184: Pirtle EC, Woods LK. Cytogenetic alterations in swine kidney cells persistently infected with hog cholera virus and propagated with and without antiserum in the medium. Am. J. Vet. Res. 29: 153-164, 1968. PubMed: 4965860

26185: Armstrong JA, et al. C-type virus particles in pig kidney cell lines. J. Gen. Virol. 10: 195-198, 1971. PubMed: 4324256

53398: Newman JT, Smith KO. Characteristics of a swine papovavirus. Infect. Immun. 5: 961-967, 1972. PubMed: 4344097

53399: Tumilowicz JJ, et al. Concurrent replication of a papovavirus and a C-type virus in the CCL 33 porcine cell line. In Vitro 15: 922-928, 1979. PubMed: 232060

56104: Todaro GJ, et al. Characterization of a type C virus released from the porcine cell line PK(15). Virology 58: 65-74, 1974. PubMed: 4132403

105588: James A House, personal communication



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯數(shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術支持:化工儀器網(wǎng)    管理登陸
亚洲精品成人片在线观看 | 久久国产亚洲欧美久久| 久久久久久人妻精品一区| 亚洲AV成人无码精品综合网站| 狠狠干| 国产精品扒开腿做爽爽爽视频| 精品久久久久久亚洲中文字幕| 亚洲色欲久久久久综合网| 国产网红无码精品福利网| 国产精品国产| 亚洲最大av在线| 亚洲伦理一区| 日韩a级| 日韩毛片在线播放| 亚洲狠狠操| 亚洲av大片| 国产无套内射普通话对白| 三上悠亚日韩精品二区| 自慰无码一区二区三区| 夜夜高潮夜夜爽国产伦精品| 色婷婷久久久SWAG精品| 亚洲色无码中文字幕手机在线| 亚洲一本之道高清乱码| 亚洲精品中文字幕无码专区| 久久久无码精品一区二区三区| 久久久免费无码成人影片| 欧洲熟妇色xxxx欧美老妇多毛| 国产成人精品成人a在线观看| 日本1区| www.喷水| 亚洲精品久久久久久久蜜桃| 激情av在线| bt电影天堂| 国产95在线 | 亚洲| 精品人妻无码中字系列| 老熟女富婆激情刺激对白| 精品久久久久久无码专区不卡 | 天天摸日日添狠狠添婷婷| 日韩精品无码熟人妻视频| 国产深夜男女无套内射| 欧美激情内射喷水高潮|