精品国产乱码一区,欧美自拍网站一二,亚洲AV熟女传媒一区二区三区,亚洲在线麻豆

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>小鼠正常細(xì)胞>CRL-2279MS1 小鼠胰島內(nèi)皮細(xì)胞

MS1 小鼠胰島內(nèi)皮細(xì)胞

簡(jiǎn)要描述:CRL-2279 MS1 小鼠胰島內(nèi)皮細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-2279
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-04-26
  • 訪  問(wèn)  量:2388

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2279 MS1 小鼠胰島內(nèi)皮細(xì)胞 的詳細(xì)介紹

CRL-2279 MS1 小鼠胰島內(nèi)皮細(xì)胞

ATCC® Number:  CRL-2279™

Designations:  MS1 (MILE SVEN 1)

Depositors:   JL Arbiser

Biosafety Level: 2 [CELLS CONTAIN PAPOVAVIRUS ]

Shipped:  froz


Organism: Mus musculus (mouse)en

Medium & Serum:  See Propagation

Growth Properties: adherent

Morphology: endothelial


CRL-2279

Source: Organ: pancreas

Strain: C57BL/6

Tissue: islet of Langerhans; endothelium

Cell Type: SV40 transformed

Cellular Products: tissue inhibitor of bioreactive matrix metalloproteinase (high levels)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Receptors: vascular endothelial growth factor (VEGF), expressed

Comments: MS1 is a pancreatic islet endothelial cell line established in 1994.

Primary islet endothelial cells were transduced with a temperature sensitive SV40 large T antigen (tsA-58-3) constructed and screened for resistance to G418.

Resistant colonies were isolated in cloning rings and screened for uptake of diI-Ac-LDL.

The line retains many properties of endothelial cells including uptake of acetylated LDL and expression of both Factor VIII related antigen and VEGF receptor.

The cells are useful for the study of signal transduction of angigenic factors.

It is the only line known that can give rise to benign hemangiomas.

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Preservation:  Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 22603: Arbiser JL, et al. Oncogenic H-ras stimulates tumor angiogenesis by two distinct pathways. Proc. Natl. Acad. Sci. USA 94: 861-866, 1997. PubMed: 9023347

51452: Arbiser JL, et al. Overexpression of VEGF 121 in immortalized endothelial cells causes conversion to slowly growing angiosarcoma and high level expression of the VEGF receptors VEGFR-1 and VEGFR-2 in vivo. Am. J. Pathol. 156: 1469-1476, 2000. PubMed: 10751370


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
欧美精品一国产成人综合久久| 在线看片人成视频免费无遮挡| 九色porny丨国产精品| 成人亚洲性情网站WWW在线观看 | 人人爽人人爽人人爽av片特级| 大香蕉久久久| 欧美精| 老四电影网| 爱在午夜降临前| 成人国产| 欧美日韩精品视频| 日本久久网站| 老色鬼在线精品视频在线观看| 在线永久免费观看黄网站| 狠狠色综合激情丁香五月| 无码人妻久久一区二区三区免费| 波多野结衣的AV一区二区三区| 亚洲处破女AV日韩精品| 人人超碰人人爱超碰国产| 2012中文字幕在线中字下载| 亚洲综合伊人久久大杳蕉| 国产在线精品一区二区高清不卡| 亚洲欧美日产综合在线网| 亚洲综合小说另类图片动图| 中文激情在线一区二区| 亚洲AV中文无码字幕色最| 久久只有这里才是精品| 亚洲中文字幕无码中文字在线| 人妻av无码系列一区二区三区| 女同亚洲一区二区无线码| 99久久久成人国产精品免费| AV无码电影一区二区三区| 无码人妻黑人中文字幕| 日产无人区一线二线三线最新版| 老司机午夜精品视频无码| 亚洲最新版av无码中文字幕一区| 免费无码又爽又刺激高潮| 国产熟女一区二区三区四区五区 | 欧美1区| 黑人操人妻| 久久综合婷婷|