精品国产乱码一区,欧美自拍网站一二,亚洲AV熟女传媒一区二区三区,亚洲在线麻豆

咨詢(xún)熱線(xiàn)

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>小鼠正常細(xì)胞>CRL-1715 TM4 正常小鼠睪丸Leydig細(xì)胞

CRL-1715 TM4 正常小鼠睪丸Leydig細(xì)胞

簡(jiǎn)要描述:CRL-1715 TM4 正常小鼠睪丸Leydig細(xì)胞,
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|;
細(xì)胞庫(kù)管理規(guī)范,
提供的細(xì)胞株背景清楚,
提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):
  • 廠(chǎng)商性質(zhì):生產(chǎn)廠(chǎng)家
  • 更新時(shí)間:2025-04-26
  • 訪(fǎng)  問(wèn)  量:2203

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

CRL-1715 TM4 正常小鼠睪丸Leydig細(xì)胞 的詳細(xì)介紹

CRL-1715 TM4 正常小鼠睪丸Leydig細(xì)胞

ATCC® Number:CRL-1715™  Price:$329.00
Designations:TM4

Depositors:JP Mather

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:epithelial


Source:Organ: testis
Disease: normal
Cell Type: Sertoli cell;


Cellular Products:retinol binding protein

tissue plasminogen activator

transferrin



Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Applications:transfection host (Roche FuGENE® Transfection Reagents)

Receptors:follicle stimulating hormone (FSH), expressed

androgen receptor, expressed

progesterone receptor, expressed



Tumorigenic:No

Antigen Expression:H-Y antigen; Mus musculus, expressed

Age:11 to 13 days

Gender:male

Comments:The TM4 cell line is reported to respond to FSH with an increase in cAMP production, but to not respond to luteinizing hormone (LH). The FSH responsiveness is much reduced compared to primary sertoli cell cultures. Constitutive plasminogen activator production is reported to be low, but is stimulated by FSH and, to a greater extent, by retinoic acid.

Tested and found negative for ectromelia virus (mousepox).



Propagation:ATCC complete growth medium: A 1:1 mixture of Ham'S F12 medium and Dulbecco's modified Eagle's medium with 1.2 g/L sodium bicarbonate and 15 mM HEPES, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell supension to new culture vessels.

  6. Incubate cultures at 37C.


Subc*tion Ratio: A subc*tion ratio of 1:10 to 1:50 is recommended
Medium Renewal: Every 3 to 4 days


Preservation:Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

recommended serum:ATCC 30-2020

recommended serum:ATCC 30-2040



References:1158: Mather JP. Establishment and characterization of two distinct mouse testicular epithelial cell lines. Biol. Reprod. 23: 243-252, 1980. PubMed: 6774781

1159: Mather JP, et al. Culture of testicular cells in hormone-supplemented serum-free medium. Ann. N.Y. Acad. Sci. 383: 44-68, 1982. PubMed: 7046561

1184: Carson DD, et al. Synthesis and secretion of a novel binding protein for retinol by a cell line derived from Sertoli cells. J. Biol. Chem. 259: 3117-3123, 1984. PubMed: 6538197

26150: Mather JP, Phillips DM. Establishment of a peritubular myoid-like cell line and interactions between established testicular cell lines in culture. J. Ultrastruct. Res. 87: 263-274, 1984. PubMed: 6544874



















產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話(huà):

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢(xún)熱線(xiàn)

400-821-8510

(周一至周日9:00- 19:00)

在線(xiàn)咨詢(xún)
  • 掃一掃 微信咨詢(xún)

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
bt天堂新版中文在线地址| 色666| 亚洲午夜无码精品福利在线看| 亚洲熟妇AV一区二区三区浪潮| 国产精品偷伦视频观看免费| 国产成人无码精品一区在线观看| 国产强伦人妻毛片| 麻花传媒MV一二三区别| 中文字幕丶东京热| 国产精品自产拍高潮在线观看| 国产精品任我爽爆在线播放| 欧美XXXX做受欧美18| 国产精品后入内射日本在线观看| 少妇高潮太爽了在线观看| 欧美婷婷六月丁香综合色| 亚洲av综合色一区二区三区| 国产精品亚洲二区在线观看| 狂野欧美激情性XXXX| 亚洲最新无码中文字幕久久| 欧洲国产精品无码专区影院| 亚洲人成网址在线播放| 无码色偷偷亚洲国内自拍| 亚洲人成网站色ww| 国产精品Ⅴ无码大片在线看| 中日韩精品视频在线观看| 国产精品欧美久久久久无广告| 成视频年人黄网站免费视频| 六月婷婷久香在线视频| 亚洲成av人在线视| 国产精品欧美亚洲韩国日本久久 | 精品国产一区二区三区久久久蜜月| 韩国三级中文字幕hd久久精品| 亚洲国产成人在线| 亚洲激情图| 夜夜操夜夜| 超碰精品99久久久久久宅男av| 国产美女一区二区| 福建论坛| 激情一区| www.久久爱白液流出h好爽| 大班冰皮月饼|