精品国产乱码一区,欧美自拍网站一二,亚洲AV熟女传媒一区二区三区,亚洲在线麻豆

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>COLO 320HSR細(xì)胞, 人結(jié)腸癌細(xì)胞

COLO 320HSR細(xì)胞, 人結(jié)腸癌細(xì)胞

簡要描述:COLO 320HSR細(xì)胞, 人結(jié)腸癌細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻和*培養(yǎng)條件

  • 產(chǎn)品型號:
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2025-04-28
  • 訪  問  量:1870

產(chǎn)品分類

Product Category

詳細(xì)介紹

COLO 320HSR細(xì)胞, 人結(jié)腸癌細(xì)胞

COLO 320DM (ATCC® CCL-220™)

Organism  Homo sapiens, human

Tissue  colon

Product Format  frozen

Morphology  rounded and refractile

Culture Properties  mixed, adherent and suspension

Biosafety Level  1

Disease  Dukes' type C, colorectal adenocarcinoma

Age  55 years

Gender  female

Ethnicity  Caucasian

Storage Conditions

Karyotype  modal number = 24; range = 49 to 61.

The stemline chromosome number is hyperdiploid and 9-11 marker chromosomes (M1, M2, M3, M5, M10, M11, M12, M13, M14, 7p- and 10 q+) were common. Double minutes were observed in 24% of the metaphases examined.

Images

Clinical Data  55 years

Caucasian

female

Genes Expressed  serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone

Tumorigenic  Yes

Effects  Yes, in nude mice

Comments  The line is negative for CEA, CSAp and colon antigen 3.

The cells are weakly positive for keratins and vimentin

Complete Growth Medium  The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.


COLO 320HSR細(xì)胞, 人結(jié)腸癌細(xì)胞

Subculturing  Shake flask, pour one-half of the medium into a new flask and add fresh medium to both flasks. Cells remaining attached may be removed (if desired) using a standard trypsin protocol.

1.Add 2.0 to 3.0 mL of 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed.

2.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

3.Add appropriate aliquots of the cell suspension to new culture vessels.

4.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:5 to 1:10 is recommended

Medium Renewal: Every 2 to 3 days

Cryopreservation  Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO

Storage temperature: Liquid nitrogen vapor phase

Culture Conditions  Temperature: 37°C

STR Profile  Amelogenin: X

CSF1PO: 11

D13S317: 11

D16S539: 11,12

D5S818: 12

D7S820: 9,12

THO1: 9

TPOX: 8,9

vWA: 15,18

Isoenzymes  ES-D, 1

G6PD, B

PEP-D, 1

PGD, A

PGM1, 1

PGM3, 2

Name of Depositor  GE Moore

References  Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117

Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

References  Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117

Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
久久91久久久久麻豆精品| AV无码久久久久久不卡网站| 精品欧美黑人一区二区三区| 国产真实乱对白精彩久久| 国产亚洲欧美精品一区| 久久久成人| 欧美激情在线狂野欧美精品 | 熟妇人妻av无码一区二区三区| 亚洲人成人网站在线观看 | 亚洲精品无码专区| 欧妇女乱妇女乱视频| 国产无遮挡无码视频免费软件| A级毛片免费| 久久久久性| 久久国产劲爆∧v内射| 久久夜色撩人精品国产| 国产色视频一区二区三区QQ号| 国模国产精品嫩模大尺度视频| 亚洲天堂免费| 久久久熟女| 广式月饼排行榜前十名| 亚洲日韩欧美在线一区二区| 日本成本人片免费网站| 精品无码久久久久国产手机版| 一级女性全黄久久生活片免费| 熟妇熟女乱妇乱女网站| 亚洲天堂2016| 亚洲精品国产a久久久久久| 精品二区| 波多野结衣AV一区二区全免费观看| 欧洲成人午夜精品无码区久久| 精品国产精品久久一区免费式 | 亚洲色欧美色2019在线| 少妇午夜福利一区二区| 久久久久人妻一区二区三区VR | 日本一级色大片| 国产自拍一区| 亚洲avwww| 三十熟女| 国产午夜福利片| 一区二区视频在线播放|