精品国产乱码一区,欧美自拍网站一二,亚洲AV熟女传媒一区二区三区,亚洲在线麻豆

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-5946NCI-H2452細(xì)胞, 人間皮瘤細(xì)胞

NCI-H2452細(xì)胞, 人間皮瘤細(xì)胞

簡要描述:NCI-H2452細(xì)胞, 人間皮瘤細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-5946
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2025-04-27
  • 訪  問  量:1848

產(chǎn)品分類

Product Category

詳細(xì)介紹

NCI-H2452細(xì)胞, 人間皮瘤細(xì)胞

ATCC® Number:CRL-5946™ Price:
Designations:NCI-H2452 [H2452]Depositors:AF Gazdar, JD MinnaBiosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiensMorphology:epithelial                         NCI-H2452細(xì)胞, 人間皮瘤細(xì)胞 Source:Disease: mesotheliomaPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.Restrictions:The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. ephone (214) 699-8056, (214) 688-7233.Isolation:Isolation date: November, 1990DNA Profile (STR):Amelogenin: X,Y CSF1PO: 11,12 D13S317: 12 D16S539: 11,13 D5S818: 11,12 D7S820: 9,11 THO1: 6,9.3 TPOX: 8,11 vWA: 17,18Gender:maleComments:The line was established in November 1990. The tissue donor was a non-smoker.Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Temperature: 37.0°CSubculturing:Protocol:Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C. Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:6 is recommended Medium Renewal: Every 2 to 3 daysPreservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phaseRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001recommended serum:ATCC 30-2020References:23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996..55657: Sekido Y, et al. Neurofibromatosis type 2 (NF2) gene is somatically mutated in mesothelioma but not in lung cancer. Cancer Res. 55: 1227-1231, 1995. PubMed: 788231355700: Penno MB, et al. High CD44 Expression on Human Mesotheliomas Mediates Association with Hyaluronan. Cancer J. Sci. Am. 1: 196-203, 1995.


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
久久男人AV资源网站无码| 欧美精品一区在线发布 | 亚洲综合区小说区激情区| 亚洲乱码日产精品BD| 久久黄色大片| 国产freesexvideos性中国| 亚洲熟妇无码另类久久久| 国产青榴视频a片在线观看| 亚洲综合色一区二区三区| 色图网| 久久天天躁狠狠躁夜夜| 熟女少妇精品一区二区| 中文精品久久久久国产| 久久久久久一区二区三区| 熟女激情| 动漫精品一区二区三区在线| 欧美日韩视频在线第一区| 亚洲精品高清国产一久久 | 九色综合婷婷| 综合久| 国产一区二区三区AV在线无码观看| 国产精品亚洲а∨无码播放| 无码人妻精品一区二区三区下载 | 女人18片毛片90分钟| 久久久久久久免费视频| 无码国产精成人午夜视频| 国产精品无码专区| 在线精品亚洲一区二区绿巨人| 免费无码午夜福利片| 久精品国产欧美亚洲色aⅴ大片| 精品一区二区三区蜜桃| 无套| asian艳丽少妇hdpics| 99热综合| 18禁亚洲| 人人爽人人干| 亚洲色婷婷六月亚洲婷婷6月| 最新国产精品剧情在线SS | 99精品国产在热久久| 国产一区二区三区不卡在线看| 鹤壁市|