精品国产乱码一区,欧美自拍网站一二,亚洲AV熟女传媒一区二区三区,亚洲在线麻豆

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人胚腎細(xì)胞>CRL-11268293T/17細(xì)胞,人胚腎細(xì)胞

293T/17細(xì)胞,人胚腎細(xì)胞

簡要描述:293T/17細(xì)胞,人胚腎細(xì)胞
,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號:CRL-11268
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-05-25
  • 訪  問  量:2334

產(chǎn)品分類

Product Category

詳細(xì)介紹

293T/17細(xì)胞,人胚腎細(xì)胞

Permits and Restrictions

View Restrictions

OrganismHomo sapiens, human
Tissuekidney
Product Formatfrozen
Morphologyepithelial
Culture Propertiesadherent
Biosafety Level2 [Cells contain Adeno and SV-40 viral DNA sequences]
Agefetus
Applications

These cells constitutively express the simian virus 40 (SV40) large T antigen, and clone 17 was selected specifically for its high transfectability.  293T/17細(xì)胞,人胚腎細(xì)胞

Storage Conditionsliquid nitrogen vapor phase
Derivation

The 293T/17 cell line is a derivative of the 293T (293tsA1609neo) cell line. 293T is a highly transfectable derivative of the 293 cell line into which the temperature sensitive gene for SV40 T-antigen was inserted. 293T cells were cloned and the clones tested with the pBND and pZAP vectors to obtain a line capable of producing high titers of infectious retrovirus, 293T/17.

Antigen Expression

SV40 T antigen

Genes Expressed

SV40 T antigen

Comments

293T/17 cells were cotransfected with the pCRIPenv- and the pCRIPgag-2 vectors to obtain the ANJOU 65 (see ATCC CRL-11269) cell line. ANJOU 65 cells were cotransfected with the pCRIPgag-2 and pGPT2E vectors to obtain the BOSC 23 (see ATCC CRL-11270) ecotropic envelope-expression packaging cell line. ANJOU 65 cells were also cotransfected with the pCRIPAMgag vector along with a plasmid expressing the gpt resistance gene to obtain the Bing (see ATCC CRL-11554)amphotropic envelope-expression packaging cell line.

Complete Growth MediumThe base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.    
SubculturingVolumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended

Medium Renewal: Every 2 to 3 days

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C          
Atmosphere: air, 95%; carbon dioxide (CO2), 5%



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
搡老女人老妇老熟女hhd小说 | 日产精品久久久久久久蜜臀| 日韩午夜精品| 亚洲精品成人久久| 少妇视频网站| 久色视频在线观看| 欧美 日韩| 亚洲精品乱码久久久久久蜜桃麻豆 | 色窝窝亚洲AV网在线观看| 永久免费AV无码网站YY| 亚洲国产区男人本色| 国产精品久久久久久妇女| 强奸乱伦影音先锋| 激情综合色五月丁香六月欧美| 久久天天躁夜夜躁狠狠85麻豆 | 欧美性猛交xxxx黑人猛交| 精品欧美| 亚洲综合无码一区二区加勒此| 丰满多水的寡妇| 久久99精品国产麻豆蜜芽| 亚洲av不卡无码国产| 亚洲精品有码在线观看| 婷婷射精av这里只有精品| 亚洲成在人线天堂网站| 亚洲一区AV无码少妇电影☆| YW尤物AV无码国产在线观看| 欧美sss| 欧美性潮喷| 熟女六十路| 类乌齐县| 亚州国产av一区二区三区伊在| 少妇中文字幕乱码亚洲影视| 色欲AV无码一区二区三区| 国产精品高清一区二区三区不卡| y111111少妇影院无码| 亚洲AV成人一区二区三区在线播放| 国产精品入口66mio女同| seseav| 九色.com| 中国性老太cd大全| 犍为县|