精品国产乱码一区,欧美自拍网站一二,亚洲AV熟女传媒一区二区三区,亚洲在线麻豆

咨詢熱線

15000266580

當前位置:首頁 >產(chǎn)品中心>細胞庫>人正常細胞>CRL-11268 293T/17人胚腎細胞

CRL-11268 293T/17人胚腎細胞

簡要描述:CRL-11268 293T/17人胚腎細胞
,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和培養(yǎng)條件

  • 產(chǎn)品型號:
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2025-04-27
  • 訪  問  量:1969

產(chǎn)品分類

Product Category

詳細介紹

CRL-11268 293T/17人胚腎細胞

Permits and Restrictions

View Restrictions

OrganismHomo sapiens, human
Tissuekidney
Product Formatfrozen
Morphologyepithelial
Culture Propertiesadherent
Biosafety Level2 [Cells contain Adeno and SV-40 viral DNA sequences]
Agefetus
Applications

These cells constitutively express the simian virus 40 (SV40) large T antigen, and clone 17 was selected specifically for its high transfectability.  CRL-11268 293T/17人胚腎細胞

Storage Conditionsliquid nitrogen vapor phase
Derivation

The 293T/17 cell line is a derivative of the 293T (293tsA1609neo) cell line. 293T is a hmperature sensitive gene for SV40 T-antigen was inserted. 293T cells were cloned anighly transfectable derivative of the 293 cell line into which the ted the clones tested with the pBND and pZAP vectors to obtain a line capable of producing high titers of infectious retrovirus, 293T/17.

Antigen Expression

SV40 T antigen

Genes Expressed

SV40 T antigen

Comments

293T/17 cells were cotransfected with the pCRIPenv- and the pCRIPgag-2 vectors to obtain the ANJOU 65 (see ATCC CRL-11269) cell line. ANJOU 65 cells were cotransfected with the pCRIPgag-2 and pGPT2E vectors to obtain the BOSC 23 (see ATCC CRL-11270) ecotropic envelope-expression packaging cell line. ANJOU 65 cells were also cotransfected with the pCRIPAMgag vector along with a plasmid expressing the gpt resistance gene to obtain the Bing (see ATCC CRL-11554)amphotropic envelope-expression packaging cell line.

Complete Growth MediumThe base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.      
SubculturingVolumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.

S

Medium Renewal: Every 2 to 3 days

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯數(shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
欧美人妖valentina| 国产VA免费精品高清在线| 国产乱| 国产精品成人观看视频| 国产精品免费无遮挡无码永久视频| 亚洲欧洲日本无在线码| 欧美黑人性暴力猛交喷水黑人巨大 | 亚洲高清一区二区三区不卡| 江津市| 蜜月av夜夜澡人人爽人人软件| 国产bbw| 99国产精品全国在线观看| 四虎国产精品成人| 蜜臀AV在线无码国产| 性荡视频播放在线视频7777| 九色综合九色综合色鬼| 亚洲精品无码专区久久| 两女女百合互慰AV赤裸无遮挡| 久久久久久久久久久大尺度免费视频| 欧美疯狂做受bbbbbb| 国产精品一区在线观看你懂的| 欧美啪啪啪| 精品伊人久久久大香线蕉下载| 亚洲AV无码专区在线| 亚洲欧美人成电影在线观看| 久久久久无码国产精品一区| 黄总轻轻挺进新婚少妇| 久9热这里有真品8| 8090yy成人免费看片| a毛片免费看| 伊人久久精品无码二区麻豆| 无码专区久久综合久综合字幕| 特婬女子婬乱视频一区二区三区| 免费无码Av片在线观看网站| 青青久在线视频免费观看| 久久久国产精品一区二区18禁| bbbbbxxxxx精品农村野外| 亚洲 日韩 另类 制服 无码| 欧美videosex性极品hd| 51ⅴ精品国产91久久久久久| 国精品产品区|