精品国产乱码一区,欧美自拍网站一二,亚洲AV熟女传媒一区二区三区,亚洲在线麻豆

咨詢熱線

15000266580

當(dāng)前位置:首頁  >  技術(shù)文章  >  4T1小鼠乳腺癌細(xì)胞

4T1小鼠乳腺癌細(xì)胞

更新時(shí)間:2022-10-21      點(diǎn)擊次數(shù):5362

細(xì)胞名稱:4T1小鼠乳腺癌細(xì)胞
介紹:4T1 是從410.4瘤株中未經(jīng)誘變篩得的6-硫鳥嘌噙抗性細(xì)胞株。 當(dāng)注射到BALB/c 小鼠中時(shí),4T1自發(fā)產(chǎn)生高轉(zhuǎn)移腫瘤,可轉(zhuǎn)移到肺,肝,淋巴結(jié)和大腦,同時(shí)在注射部位形成始發(fā)灶。 誘導(dǎo)轉(zhuǎn)移時(shí)不需要摘除始發(fā)灶。

4T1小鼠乳腺癌細(xì)胞在BALB/c小鼠中的生長與轉(zhuǎn)移特性與人體中的乳腺癌十分相近。這種腫瘤是人VI期乳腺癌的動(dòng)物模型。 4T1-誘導(dǎo)的腫瘤在手術(shù)后及未手術(shù)情況下轉(zhuǎn)移的動(dòng)力學(xué)相近,可以用作手術(shù)后及未手術(shù)模型。 跟其他腫瘤模型相比,由于4T1的抗6-硫鳥嘌噙特性,微小的轉(zhuǎn)移細(xì)胞團(tuán)(少到僅僅1個(gè))也可以在許多遠(yuǎn)端器官中檢測到。

沒必要數(shù)淋巴結(jié)或稱重器官

形態(tài)特性       上皮細(xì)胞
生長特性      貼壁生長
傳代方法       消化3-5分鐘。1:2。3天內(nèi)可長滿。
支原體檢測      陰性

下面是ATCC的介紹

4T1 (ATCC® CRL-2539™)
Organism    Mus musculus, mouse
Tissue     mammary gland
Product Format     frozen4T1 小鼠乳腺癌細(xì)胞
Morphology     epithelial
Culture Properties     adherent
Biosafety Level     1
Disease     This tumor is an animal stage IV human breast cancer.
Strain     BALB/cfC3H
Applications    
The tumor growth and metastatic spread of 4T1 cells in BALB/c mice very closely mimic human breast cancer. This tumor is an animal model for stage IV human breast cancer.

4T1-induced tumors can be used as a post-operative model as well as a non-surgical model because the 4T1-induced tumor metastasizes spontaneously in both models with similar kinetics.
Storage Conditions     liquid nitrogen vapor temperature
Derivation    
4T1 is a 6-thioguanine resistant cell line selected from the 410.4 tumor without mutagen treatment.
Tumorigenic     Yes
Effects    4T1小鼠乳腺癌細(xì)胞
Yes, forms tumors and metastasizes in BALB/c mice
Comments    
When injected into BALB/c mice, 4T1 spontaneously produces highly metastatic tumors that can metastasize to the lung, liver, lymph nodes and brain while the primary tumor is growing in situ.
The primary tumor does not have to be removed to induce metastatic growth.
Because 4T1 is resistant to 6-thioquanine, micro-metastatic cells (as few as 1) can be detected in many distant site organs with better accuracy that most tumor models. There is no need to count nodules or weight target organs.

Complete Growth Medium     The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Phenol Red is a structural mimic for estrogen. Therefore the estrogenic activity of phenol red should be considered in any studies that utilize estrogen-responsive cells in culture (Ref). The Phenol Red-free version of RPMI 1640, is ATCC catalog No. 30-2602.

Subculturing    4T1小鼠乳腺癌細(xì)胞
NOTE: the cells should not be allowed to become confluent, subculture at 80% of confluence. Remove medium, and rinse with 0.25% trypsin-0.53mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subc*tion Ratio: A subc*tion ratio of 1:6 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation    
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions    
Temperature: 37°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
欧洲美熟女乱又伦av影片| 久久久久亚洲AV无码去区首| 亚洲av手机在线观看不卡| 精品国产三级a∨在线观看| 国产一级特黄录像片| 日躁夜躁狠狠躁2001| 97网站| 两性色午夜免费视频| 亚洲国产成人aV毛片大全a| 中文字幕无码乱人伦在线| 久久精品人人做人人综合试看| 18禁裸乳无遮挡啪啪无码免费| JZZIJZZIJ日本成熟少妇| 一本一道VS无码中文字幕| 亚洲区小说区图片区QVOD| 中文字幕丰满乱孑伦无码专区| 亚洲自偷自拍熟女另类| 五月婷色| 国内少妇情人精品av| 性中国熟妇| 久草成人| 一区二区三区美女| 亚洲精品国产精品乱码不66| 亚洲综合区夜夜久久久| 国产偷v国产偷v亚洲高清| 天堂√在线中文最新版8| 亚洲精品国产肉丝袜久久| 精品亚洲成在人线AV无码| 精品国产AV最大网站| 亚洲人成小说网站色在线| 国产无遮挡又黄又爽在线观看| 欧美亚洲日产综合新一区| 高清视频大片免费观看| 亚洲人成无码区在线观看| 一本久久A久久免费精品不卡 | 丰满少妇被猛烈进入| 欧洲s码亚洲m码精品一区| 亚洲日产无码中文字幕| 国产成人综合色就色综合 | 欧美在线色| 丰满少妇一区二区三区|